Assessment of Serum Ferritin Levels and Hematologic Changes in Blood Donors: Impact of Donation Frequency

Abstract

Background: Blood donation is a vital component of modern healthcare systems, supporting medical 
procedures and emergency responses by providing life-saving blood components.
Materials and Methods: A cross-sectional study was conducted at a tertiary hospital with 120 voluntary 
blood donors aged 18-60 years, including first-time and repeat donors. Data collection involved 
demographic details and categorization of donation frequency into first-time, infrequent (less than twice a 
year), and frequent (two or more times a year). Blood samples were collected to measure serum ferritin 
using enzyme-linked immunosorbent assay (ELISA) and to perform a complete blood count (CBC) 
assessing hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular 
hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC).