EXPLORING UV-INDUCED DNA DAMAGE AND ITS ROLE IN SKIN CANCER PATHOPHYSIOLOGY

Abstract

Testing mammalian cells' capacity to repair UV-damaged DNA has been made feasible using
UV-damaged viruses and genes. The non-replicating recombinant adenovirus Ad5HCMV
space, specifically, has been employed to study constitutive and inducible repair mechanisms
of UV-damaged DNA in human cells, utilizing the B-galactosidase reporter gene. Human
fibroblasts exposed to low UV fluences were previously treated with UV light, temporarily
enhancing host cell reactivation to produce the UV-damaged reporter gene.