Agricultural wastes as substrates and immobilization of spores to increase lactic acid production by Rhizopus oryzae ATCC 4858
DOI:
https://doi.org/10.48047/Keywords:
Rhizopus oryzae ATCC 4858, CBS 327.47, DSM 853, lactic acid, agricultural wastes, immobilization, CMC pellets, total titratable acidity.Abstract
The present work explored L-lactic acid (LA) produced from agro-wastes by spores of Rhizopus
oryzae ATCC 4858 by native hydrolysis prospective of the present work organism.
Immobilization of spores on agar-agar, carboxymethyl cellulose sodium salt (Na-CMC), artificial
fiber sponge, loofa sponge or maize stalks pulp was also investigated. Rice straw gave maximum
amount of LA by the fungus. Descending sequence of agricultural wastes according to LA amounts
by the fungusis as follows: rice straw > pomegranate peel > orange fruits peel > bagasse > eggplant
fruits peel > soya bean meal > wheat bran. Highest amount of lactic acid was produced using
spores carried on synthetic sponge than loaf cubic pieces then CMC pellets then pellets of agaragar and finally cubic pieces of corn stalk pith hemicelluloses. TTA values were the least at agaragar pellets and maximum at cubic pieces. Final pH value (more acidic) was detected in case of
synthetic sponge as a carrier, while the highest was detected on pellets of CMC
