FAVIPIRAVIR-ANALYTICAL METHODS
DOI:
https://doi.org/10.48047/Keywords:
Favipiravir, antiviral, HPLC method, spectrofluorimetric method.Abstract
Favipiravir a pyrazine analogue its shows antiviral activity of viruses. The separation was carried out by C18 column.
The mixture of potassium dihydrogen phosphate (mobile phase A) and acetonitrile (mobile phase B) for flowing
phase. In HPLC column temperature was 30° and ultraviolet detection was 323nm. The column run time was 15min
under chromatographic conditions. Excellent linear relationship between peak area and favipiravir concentrations has
been observed. Developed HPLC method found to be sensitive, precise, accurate, specific and robust. Proposed
method has successfully applied for quantification of favipiravir in pharmaceutical formulations.
Spectrofluorimetric method for determination of favipiravir. Different factors effecting fluorescence were carefully
studied. The proposed method is based on measuring native fluorescence of favipiravir IN 0.2 M borate buffer at 432
nm after at 361nm. Relationship between favipiravir concentration and relative fluorescence intensity. The method
was successfully implemented for determination of favipiravir in its pharmaceutical formulation.
