Therapeutic Efficacy of Adipose-Derived Mesenchymal Stem Cells after Chronic Fluoxetine Treatment on pars nervosa in adult albino rats (light and immunohistochemical study)

Abstract

Background:Fluoxetine (FLU) is a prototype of SSRIs and a first line drug for the treatment of
depression. Aim of the work:This study was planned todemonstrate the histological features of pars
nervosa after chronic fluoxetine treatment and the possible therapeutic effect of adipose-derived
mesenchymal stem Cells.Material and Method: Thirty healthy male adult albino ratswere classified
into four groups. Control group(Group I) included fifteen rats. Fluoxetine treated (Group II) included
five rats that received 24 mg/kg/day of fluoxetine dissolved in 1.0 ml of tap water once a day.
Fluoxetine group treated with ADSCs (Group III)included five rats that received fluoxetine as group
(II) for30 days then injected once by ADSCs at a dose of 1×106
 cells/rat in the tail vein suspended in
0.5ml of phosphate-buffered saline (PBS). Recovery group (Group IV) included five rats that received
fluoxetine for 30 days then received no treatment till the end of the experiment. Samples from pars
nervosa were processed for light,immunohistochemical, morphometrical and statistical analyses.
Results: Fluoxetine treated group showed pituicytes with different shaped nuclei and pale or
vacuolated cytoplasm. Acidophilic small Herring bodies were noticed. Administration of ADSCs
greatly improved microscopic appearance ofcellswhile, recovery group showed more or less similar
histological changes as Fluoxetine group. Conclusion: Fluoxetine induced various deleterious changes
on the pars nervosa of albino rat. These changes were almost corrected by ADSCs treatment.