PHYTOCHEMICAL SCREENING & IN-VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY FROM STEM EXTRACT OF PLANT PIPER BETEL

Abstract

The objective of the present project work was to investigate the active constituent of plant Piper betel
characterized for its antimicrobial activity. For the research analysis the stem powder subjected to phytochemical
screening and was found to contain carbohydrate, protein, polyphenolic compounds, flavonoid, alkaloids and total
antioxidant. The estimation and evaluation of antimicrobial activity was done by disc diffusion method. For this the
different concentration ethanolic extract (20, 40, 60 & 80%) was prepared and from which initial concentration of
1mg/ml were loaded on 6mm dimension filter paper which was sterilized previously by autoclaving. The loaded disc
was placed on surface of medium and the compound was allowed to diffuse for 5 minutes and all the plated were
incubated at 37℃ for 24 hrs. The end of incubation, inhibition zones formed around the disc in few mm,
measurement done by using the ruler. As, a result the maximum inhibition was showed by the concentration of 60%
dilution. The extract confirmed significant antimicrobial activity against the bacterial strains test.