Morphological Evaluation Of The Diagnostic Efficacy Of Cytological Smear Technique Versus Cell Block Method In The Cytology Of Body Fluids
DOI:
https://doi.org/10.48047/Keywords:
Conventional smear, Cell block, Body fluids This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution‑ Non Commercial‑ Share Alike 4.0 License, which allows others to remix, tweak, and build upon the work non‑ commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.Abstract
Background: The present study was conducted for evaluating body fluids with conventional smear and cell block at tertiary
care hospital. Materials & methods: The present study was conducted on 50 body fluid samples (pleural, synovial, ascitic
fluid) from patients were analysed in the cytopathology laboratory of our institute.All 50 fluid specimens were examined for
conventional cytological smear (CS) and cell block method (CB). Each fluid specimen was divided in two equal portions
with one portion subjected to conventional smear technique, Smear was immediately fixed with 95% alcohol and stained
with Haematoxylin-Eosin (H&E). The other part was subjected to 10% alcohol-acetic acid-formalin cell block technique.
Paraffin- embedded 4-6 μ thick sections were routinely stained with H&E.The morphological characteristics, cellularity,
nuclear and cytoplasmic details were evaluated in both CS and CB techniques. Results:10 cases were malignant positive for
both smear and cell block, 5 cases were suspicious for malignancy for smear and were positive for malignancy on evaluation
in cell block, 6 cases of mesothelial cluster which were positive for malignancy in conventional smear were negative on
evaluation from cell block, 29 cases were benign on evaluation from both smear and cell block. Conclusion: The study
found that methods had high specificity. Cell- blocks were found to be more reliable to smears in typing malignancy.
