Effect of Different Intracanal Medicaments on the Viability and Survival of Dental Pulp Stem Cells

Abstract

Background: Regenerative endodontic techniques rely heavily on stem cells for success. 
They are impacted by medications that are taken prior to creating a scaffold for endodontic regeneration 
or inducing bleeding. The effects of several intracanal medications on the survival and viability of dental 
pulp stem cells at varying dosages and exposure durations are investigated in this work. Procedures: 
Dental pulp stem cells were obtained from healthy third molar teeth using the long-term explant culture 
method. They were then subjected to varying concentrations of calcium hydroxide, doxycycline, 
potassium iodide, triamcinolone, and glutaraldehyde, ranging from 0 (control) to 1000 µg/mL. The cells 
were then characterized using flow cytometry. Hours of exposure were 6, 24, and 48. In order to 
determine cell viability, the MTT assay, and the Annexin V-binding assay was used to quantify 
apoptosis. Findings: Cell viability was considerably decreased by all medications at varying doses and 
exposure durations.