DEVELOPMENT AND VALIDATION OF SIMPLE SPECTROSCOPIC METHODS FOR THE ESTIMATION OF HEPATITIS-C DRUG DACLATASVIR DI HYDROCHLORIDE IN PURE FORM
DOI:
https://doi.org/10.48047/Keywords:
Daclatasvir (DAC), Spectrophotometry, FC reagent, Methyl orange, Cresol red.Abstract
Three simple, sensitive, rapid and accurate spectrophotometric methods have been proposed for the estimation of
daclatsvir di hydrochloride (Dimethyl N,N'-([1,1'-biphenyl]-4,4'-diylbis{1H-imidazole-5,2-diyl-[(2S)-pyrrolidine2,1-diyl][(2S)-3-methyl-1-oxobutane-1,2-diyl]})dicarbamate) in pure form. These methods were based on the
measurement of absorbance of FC reagent [METHOD-A], Methyl orange [METHOD-B] and Cresol red
[METHOD-C] at 553nm, 425nm and 432nm respectively. Reaction conditions have been optimized to obtain
coloured complexes of higher sensitivity and longer stability. The absorbance increased linearly with increase in
concentrations of daclatasvir.The complexes obeyed Beer’s law over the concentration range of 25 - 125 µg/mL, 30 -
70 µg/mL and 30 - 70 µg/mL in method A, method B and method C respectively.The common excipients and
additives did not interfere in their determination. The developed methods have been successfully applied for the
estimation of daclatasvir in bulk samples
