DEVELOPMENT AND VALIDATION OF VISIBLE SPECTROSCOPY METHOD FOR THE DETERMINATION OF THIAMINE (VITAMIN B1)IN BULK AND PHARMACEUTICAL DOSAGE FORM

Abstract

A simple spectrophotometric method has been described for the determination of thiamine. The method is based
on the derivatization of thiamine with MBTH (0.2%w/v) in alkaline medium 2 mLNaoH (1N) was performed.
It was found that the product is Yellowcolored exhibiting λmax at 434nm.The λmax of thiamine-MBTH derivative
was red-shifted, eliminating any potential interference. The wavelength 434 nm therefore was fixed as
optimum.Beer's law was obeyed over the concentration range from 1‐50 µg/mL thiamine. The relative standard
deviation,< 1.5%;correlation coefficient,0.9998; molar absorptivity0.96x10 4
,Sandels sensitivity 35ng/cm2 ;The
limit of detection, 0.0076 µg/mL; the limit of quantification, 0.0231 µg/mL and recovery, 101.47% thiamine.
Finally, the developed method was applied to the determination of thiamine in pharmaceutical formulations.