INVITRO DRUG INTERACTION STUDY OF ALOGLIPTIN BESYLATE WITH TELMISARTAN ANDAZILSARTAN BY EQUILIBRIUM DIALYSIS USING UVSPECTROSCOPIC METHOD
DOI:
https://doi.org/10.48047/Keywords:
Dipeptidyl peptidase-4 inhibitor, Angiotensin-2 receptor blocker, Bovine serum albumin, Human serum albumin.Abstract
Introduction :This study mainly aimto investigate In vitro protein binding and drug displacement study of
Alogliptin Besylate (ALO) with the selected Angiotensin II Receptor Blockers (TEL and AZIL) using equilibrium
dialysis by UV spectroscopic method.
Method: To study the protein binding and drug displacement property of ALO with selected sartans by using the
equilibrium dialysis sac method. The effect of TEL and AZIL on in vitro protein binding of ALO was studied at
different ratios (BSA: ALO:TEL/AZIL, 1:1:1,1:1:2,1:1:3,1:1:4). And this procedure is repeated vice versa by adding
Alogliptin into increasing concentration to get a final ratio (BSA:TEL/AZIL:ALO, 1:1:1,1:1:2,1:1:3,1:1:4)for study
the effect of ALO on in vitro protein binding of TEL and AZIL. Then responses of free drug concentrations were
measured. The possibility of protein-drug binding interaction is confirmed by using Molecular Docking Studies.
Result: The specific site of protein binding of the drugs was confirmed by molecular docking studies. The
percentage protein binding of ALO, TEL and AZIL was found to be ≤ 21.4%, ≤95.5%, and ≤93.2% respectively.
The displacement property of each drug was studied.
Conclusion: There are no drug-drug interactions are occurring between the ALO and selected sartans but as
compared to AZIL and TEL with ALO, AZIL is the more preferable choice for alogliptin when they use together.
