Antioxidant Potential of Kersen LeavesSteeping (Muntingia calabura L.) Against Endogenous Enzyme Superoxide Dismutase (SOD) Levels in Diabetes Mellitus Rats

Abstract

Oxidative stress occurs when the levels of free radicals and antioxidants in the body is not balanced. Free
radicals can be formed as a result of an increase in blood glucose levels in diabetes mellitus that can cause
damage to cells, tissues, and organs such as the liver, kidneys, heart. Antioxidants are necessary to dampen the
negative effects of oxidants. Flavonoids on the cherry crop is antioxidative. This study was investigated the
effectiveness of steeping cherry leaves (Muntingia calabura L.) in increasing levels of the enzyme SOD in
Diabetes Mellitus Rats.The subjects of this study were 36 Sprague Dawley rats divided into 6 groups, namely
group 1 (normal), group 2 (negative control), group 3 (positive control), group 4 (steeping cherry leaves 250
mg/200 grBW), group 5 (steeping kersen leaves 500 mg/200 grBW), and group 6 (steeping cherry leaves 750
mg/200 grBW). Groups 2-6 were induced with streptozotocin and nicotinamide for 5 days until the rats became
Diabetes Mellitus (blood glucose>135 mg/dl) and then treated for 14 days. Data were analyzed using a paired ttest and One-Way ANOVA test. Statistical test results with paired t-tests showed significant differences in blood
glucose levels before and after treatment (p=0.0001). In the One-Way ANOVA test, there was a different mean
of SOD levels in each group (p=0.0001). The most effective to increase SOD levels is a dose of 750 mg/200
grBW.