Formulation and Evaluation of Andrographis paniculata Topical gel for Wound healing activity

Abstract

The present study aimed to formulate the topical gel of Andrographis paniculata (A.paniculata)
extract and to evaluate the gel for its physical properties, in-vitro permeation, and wound healing
properties in rats. Materials and Methods: Topical gels were prepared using two polymers namely
Carbopol and Hydroxypropyl Methyl Cellulose (1-1.5%). The gels were evaluated for their
viscosity, spreadability, content uniformity. In-vitro diffusion study was conducted for topical gels
using Franz diffusion cell for the biomarker compound β-sitosterol. Results and Discussion: It was
observed that with an increase in the polymer percentage, there was a proportional increase in the
viscosity and reduced spreadability. Similarly, the rate of diffusion of the drug from the gels was
also reduced with an increase in the polymer concentration. All the prepared formulations
demonstrated content uniformity and moderate to good spreadability. Treatment with Topical gel
decreased wound contraction time. The fall of eschar left no raw wound behind (0.397±0.675).
Percentage wound contraction (96.22) in 14-15 days, the results of the wound healing activity were
significantly better than the control group. Conclusions: It can be concluded that A. paniculata
loaded Topical gels is a viable option for the treatment of wounds.