The effect of immunizing laboratory rabbits with O-antigen purified from Escherichia coli and infected experimently with Giardia lamblia on some Cytokines

Abstract

This study was conducted for the period from January 2022 until the end of December 2022 at Samarra University / Graduate Studies Laboratory of the College of Applied Sciences, Methods: 50 New Zealand white rabbits with weight ranged from (1500-1800) gm obtained from the National Center for Drug Control and Research in Baghdad, while their ages ranged from (10-14) months.The present study included the effect of Outer Membrane Proteins (OMPs) or (O-Antigen) extracted from Escherichia coli on the immune response in male rabbits infected with G. lamblia parasite, by measuring the levels of cytokines (IL-4, IL-6 and IL-10). Results: The cytokines under study, its level increased in the serum of laboratory rabbits, and our study showed that the level of IL-4 for the O-antigen group increased significantly compared with the negative control group, where its average of concentration was (12.35 and 8.71) pg/ml, respectively. An increase in the level of IL-4 occurred in Challenge group, compared to the positive control, where its average of concentration reached (14.11 and 10.98) pg/ml, respectively.