Detection of metallo Beta Lactamase (MBl) Among clinical isolates pseudomonas aeruginosa in a tertiary care Hospital, CIMS Bilaspur [CG]

Abstract

Background: Metallo-Beta Lactamase (MBL) production in Pseudomonas aeruginosa presents a significant public health concern due to the enzyme's capacity to hydrolyze a broad range of betalactam antibiotics, including carbapenems. This resistance mechanism complicates treatment options and poses a challenge in clinical management. Objective: This study aimed to detect MBL production among clinical isolates of Pseudomonas aeruginosa at CIMS Bilaspur and to compare the effectiveness of different phenotypic detection methods. Methods: A total of 263 non-fermentative Gram-negative bacilli isolates were collected from various clinical specimens, such as pus, urine, blood, and body fluids. The isolates were screened for MBL production using the Imipenem disk diffusion method. Confirmatory testing was conducted using the Disk Potentiation Test (DPT) and E-test.