STUDY ON RELIABILITY OF SCREENING TESTS AGAINST HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR DETECTION OF SICKLE CELL DISEASE ALONG WITH ANALYSIS OF HEMOGRAM PARAMETERS AT A TERTIARY CARE HOSPITAL IN WESTERN INDIA

Abstract

Background: Sickle cell disease (SCD) and its variants are genetic disorders are due to presence of an abnormal form of haemoglobin, haemoglobin S (HBS). The role of Laboratory in the Diagnosis of Haemoglobinopathies is very important. Peripheral blood film method, sickling and sickle solubility tests are used as screening method of sickle cell disease. While High Performance Liquid Chromatography (HPLC) is confirmatory test.Aim of the study: This study was done to evaluate various types of haemoglobins and their relative percentage in sickle cell anaemia cases. Besides this, we analysed haemogram parameters such as haemoglobin (Hb), WBC, Platelets etc. Along with this we determined reliability of various screening test against HPLC technique.Method: We analysed blood samples from 95 patients suspected to have sickle cell haemoglobinopathies and all were tested for sickling screening test. All positive cases will be subjected to HPLC to separate constituent haemoglobins and complete blood count (CBC) analysis was done to check haemogram parameters