Phenotypic Characterization Of Vancomycin-Resistant Enterococcus Isolates From Different Clinical Samples At A Rural Tertiary Care Hospital: A Significant And Relevant Research In The Field Of Microbiology

Abstract

Vancomycin-resistant Enterococci (VREs) have increasingly become a major nosocomial pathogen worldwide. The increasing prevalence of vancomycin-resistant enterococci (VRE)
in hospitals poses a serious threat to patients due to their multiple drug resistance. This study
aimed to determine the prevalence of vancomycin resistance among the various Enterococcal
species isolated from clinical samples,
Methods: A Total of 150 enterococcus species isolated from different clinical samples were
subjected to vancomycin-resistant enterococcus (VRE) screening using conventional
microbiological methods. Antibiotic susceptibility testing of allenterococcalisolates and
screening and confirmatory tests were performed to detect specific resistance mechanisms.
Minimum inhibitory concentrations (MIC) of vancomycin were determined using the
microdilution method.
Results: out of 150 enterococcus species isolated from different clinical samples were subjected to vancomycin resistance using conventional microbiological methods. The
incidence rate of male VRE was 7/11 (63.6%), and female VRE was 4/11 (34.4%). Most
VREs were from male patients of different age groups. Of these, 7 were E. faecalis& 4 were
E. faecium.
Conclusion: Enterococci have emerged as a pathogen associated with severe nosocomial
infections &E. faecalis and E. faecium cause most clinical infections.